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CoraLite®594-conjugated MPO Monoclonal antibody

MPO Monoclonal Antibody for IF

Host / Isotype

Mouse / IgA

Reactivity

human, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4C11F6

Cat no : CL594-66177

Synonyms

MPO, myeloperoxidase



Tested Applications

Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66177 targets MPO in IF applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen MPO fusion protein Ag17564
Full Name myeloperoxidase
Calculated molecular weight 745 aa, 84 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC130476
Gene symbol MPO
Gene ID (NCBI) 4353
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Form Liquid
Purification Method Thiophilic affinity chromatograph
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL594 MPO antibody CL594-66177Download protocol
Standard Protocols
Click here to view our Standard Protocols