Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, MCF-7 cells, Raji cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17006-1-AP targets MRPS15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10702 Product name: Recombinant human MRPS15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-257 aa of BC031336 Sequence: MLRVAWRTLSLIRTRAVTQVLVPGLPGGGSAKFPFNQWGLQPRSLLLQAARGYVVRKPAQSRLDDDPPPSTLLKDYQNVPGIEKVDDVVKRLLSLEMANKKEMLKIKQEQFMKKIVANPEDTRSLEARIIALSVKIRSYEEHLEKHRKDKAHKRYLLMSIDQRKKMLKNLRNTNYDVFEKICWGLGIEYTFPPLYYRRAHRRFVTKKALCIRVFQETQKLKKRRRALKAAAAAQKQAKRRNPDSPAKAIPKTLKDSQ Predict reactive species |
| Full Name | mitochondrial ribosomal protein S15 |
| Calculated Molecular Weight | 257 aa, 30 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC031336 |
| Gene Symbol | MRPS15 |
| Gene ID (NCBI) | 64960 |
| RRID | AB_2301068 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P82914 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MRPS15, also named as RPMS15 or DC37, is a 257 amino acid protein, which belongs to the ribosomal protein S15P family. MRPS15 localizes in the Mitochondrion and is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. MRPS15 is involved into the mitochondrial proteins biosynthesis and ribosomal biogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MRPS15 antibody 17006-1-AP | Download protocol |
| IHC protocol for MRPS15 antibody 17006-1-AP | Download protocol |
| IP protocol for MRPS15 antibody 17006-1-AP | Download protocol |
| WB protocol for MRPS15 antibody 17006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MRPS15 Polyclonal antibody (Cat# 17006-1-AP) has 17 citations. These appear in journals including Cell, Cell Metabolism, Molecular Cell, Nucleic Acids Research, EMBO Journal, Cell Reports, JBC, Human Molecular Genetics, PLoS One, Biochimica et Biophysica Acta, Methods in Molecular Biology, and bioRxiv. Research fields span mitochondrial ribosome biogenesis, mitochondrial translation, mitoribosome composition, mitochondrial nucleoid structure, mitochondrial gene expression, mitochondrial protein synthesis, liver cancer metabolism, and mitochondrial-related genetic disorders.
| Species | Application | Title |
|---|---|---|
Cell Metab Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids. | ||
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
EMBO J Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism |
