MRPS15 Polyclonal antibody

MRPS15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 17006-1-AP

Synonyms

S15mt, RPMS15, MRP-S15, MRP S15, MPR S15

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Validation Data Gallery

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  WB analysis using 17006-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17006-1-AP (MRPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17006-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17006-1-AP (MRPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17006-1-AP

  IP result of anti-MRPS15 (IP:17006-1-AP, 4ug; Detection:17006-1-AP 1:300) with mouse brain tissue lysate 3200 ug.

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  IHC staining of human spleen using 17006-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17006-1-AP (MRPS15 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17006-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17006-1-AP (MRPS15 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 17006-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPS15 antibody (17006-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, MCF-7 cells, Raji cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below

Product Information

17006-1-AP targets MRPS15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPS15 fusion protein Ag10702
• Full Name: mitochondrial ribosomal protein S15
• Calculated Molecular Weight: 257 aa, 30 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC031336
• Gene Symbol: MRPS15
• Gene ID (NCBI): 64960
• RRID: AB_2301068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPS15, also named as RPMS15 or DC37, is a 257 amino acid protein, which belongs to the ribosomal protein S15P family. MRPS15 localizes in the Mitochondrion and is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. MRPS15 is involved into the mitochondrial proteins biosynthesis and ribosomal biogenesis.

Publications

Species	Application	Title
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza
human	WB	Cell; Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.; Authors - Ricarda Richter-Dennerlein
human	WB	Mol Cell; Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria; Authors - Sabine Poerschke
human	WB	Cell Metab; Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.; Authors - Daniel F Bogenhagen
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Hum Mol Genet; Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.; Authors - Juliette Pulman