MSI1 Polyclonal antibody

MSI1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG







Cat no : 27185-1-AP


MSI1, Musashi 1, musashi homolog 1 (Drosophila)

Tested Applications

Positive WB detected inHEK-293 cells, Caco-2 cells, HEK-293T cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27185-1-AP is MSI1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MSI1 fusion protein Ag26042
Full Name musashi homolog 1 (Drosophila)
Calculated Molecular Weight 362 aa, 39 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC146463
Gene Symbol MSI1
Gene ID (NCBI) 4440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary.


Product Specific Protocols
WB protocol for MSI1 antibody 27185-1-APDownload protocol
IHC protocol for MSI1 antibody 27185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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