MT1A Polyclonal antibody

• 

  IHC staining of human kidney using 27115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27115-1-AP (MT1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27115-1-AP (MT1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 27115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27115-1-AP (MT1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 27115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MT1A antibody (27115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

MT1A Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 27115-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

metallothionein 1A, Metallothionein IA, MT 1A, MT IA, MT1, MT1A, MT1S, MTC

---

Tested Applications

• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27115-1-AP targets MT1A in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25747

  Product name: Recombinant human MT1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-45 aa of BC029475

  Sequence: MDPNCSCATGGSCTCTGSCKCKECKCNSCKKSCCSCCPMSCAKCA

• Full Name: metallothionein 1A
• Calculated Molecular Weight: 61 aa, 6 kDa
• Observed Molecular Weight: 6 kDa
• GenBank Accession Number: BC029475
• Gene Symbol: MT1A
• Gene ID (NCBI): 4489
• RRID: AB_3669583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04731
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.