Various lysates were subjected to SDS PAGE followed by western blot with 27832-1-AP (MTSS1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 27832-1-AP (MTSS1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 27832-1-AP
IP result of anti-MTSS1L (IP:27832-1-AP, 4ug; Detection:27832-1-AP 1:300) with Jurkat cells lysate 4000 ug.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27832-1-AP (MTSS1L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 27832-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1L antibody (27832-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1L antibody (27832-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 27832-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27832-1-AP (MTSS1L antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27832-1-AP (MTSS1L antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293T cells, HeLa cells, Jurkat cells, U-251 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected in
Jurkat cells
Positive IHC detected in
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
MTSS2, also known as MTSS1L, contains a conserved N-terminal domain, called an IRSP53 /MIM homology domain (IMD) or inverse BAR domain, that is involved in plasma membrane dynamics (PMID: 20875796). MTSS2 potentiated PDGF-mediated formation of membrane ruffles and lamellipodia in fibroblasts, acting via RAC1 activation (PMID:14752106). MTSS2 is implicated in intellectual developmental disorders with ocular anomalies and distinctive facial features(PMID: 36067766).
Various lysates were subjected to SDS PAGE followed by western blot with 27832-1-AP (MTSS1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 27832-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27832-1-AP (MTSS1L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 27832-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27832-1-AP (MTSS1L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of Jurkat using 27832-1-AP
IP result of anti-MTSS1L (IP:27832-1-AP, 4ug; Detection:27832-1-AP 1:300) with Jurkat cells lysate 4000 ug.
IF/ICC Figures
IF Staining of MCF-7 using 27832-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1L antibody (27832-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 27832-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27832-1-AP (MTSS1L antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
MTSS1L Polyclonal antibody
Catalog Number
27832-1-AP
Citations
2
Dilutions
WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
