MYH10 Polyclonal antibody
MYH10 Polyclonal Antibody for FC, IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 21403-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain |
Positive IP detected in | mouse brain tissue |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 21403-1-AP is MYH10 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MYH10 fusion protein Ag16113 |
Full Name | myosin, heavy chain 10, non-muscle |
Calculated molecular weight | 1985 aa, 230 kDa |
Observed molecular weight | 229 kDa |
GenBank accession number | BC150634 |
Gene symbol | MYH10 |
Gene ID (NCBI) | 4628 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MYH10 antibody 21403-1-AP | Download protocol |
IF protocol for MYH10 antibody 21403-1-AP | Download protocol |
IP protocol for MYH10 antibody 21403-1-AP | Download protocol |
FC protocol for MYH10 antibody 21403-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |