MYL7 Polyclonal antibody

MYL7 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17283-1-AP

Synonyms

MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a



Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17283-1-AP targets MYL7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL7 fusion protein Ag11058
Full Name myosin, light chain 7, regulatory
Calculated molecular weight 175 aa, 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC027915
Gene symbol MYL7
Gene ID (NCBI) 58498
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.

Protocols

Product Specific Protocols
WB protocol for MYL7 antibody 17283-1-APDownload protocol
IHC protocol for MYL7 antibody 17283-1-APDownload protocol
IF protocol for MYL7 antibody 17283-1-APDownload protocol
IP protocol for MYL7 antibody 17283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Rep

High content analysis identifies unique morphological features of reprogrammed cardiomyocytes.

Authors - Matthew D Sutcliffe
humanWB

Stem Cell Reports

Mitochondrial Dysfunctions Contribute to Hypertrophic Cardiomyopathy in Patient iPSC-Derived Cardiomyocytes with MT-RNR2 Mutation.

Authors - Shishi Li
humanIF

Cardiovasc Res

Angiopoietin-1 enhanced myocyte mitosis, engraftment, and the reparability of hiPSC-CMs for treatment of myocardial infarction.

Authors - Zhonghao Tao
mouseIF

Nat Commun

High-efficiency reprogramming of fibroblasts into cardiomyocytes requires suppression of pro-fibrotic signalling.

Authors - Yuanbiao Zhao