Recombinant Mouse CD3 Delta protein (rFc Tag)

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• Recombinant Mouse CD3 Delta protein (rFc Tag) (Eg4665)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4665

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD3 Delta was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD3 Delta protein Phe22-Ala105 (Accession# P04235) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 12500
• Accession: P04235
• PredictedSize: 34.9 kDa
• SDS-PAGE: 40-58 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD3 Delta (CD3δ) is a key signal transduction subunit of the T cell receptor (TCR) complex and a member of the immunoglobulin superfamily. It forms the TCR-CD3 complex together with CD3ε, CD3γ, and CD3ζ. CD3δ contains an immunoreceptor tyrosine-based activation motif (ITAM). After the TCR recognizes the MHC-peptide complex, the ITAM is phosphorylated by Src family kinases, recruiting downstream signaling molecules such as ZAP-70 and initiating a cascade of signals leading to T cell activation, proliferation, and cytokine production. Mutations in the CD3δ gene lead to severe combined immunodeficiency (SCID), with patients exhibiting arrested T cell development and severely impaired immune function. Furthermore, anti-CD3 monoclonal antibodies (e.g., OKT3) target the CD3ε/δ complex to inhibit T cell activation and are widely used in the treatment of organ transplant rejection and autoimmune diseases. In immunological research and clinical diagnosis, anti-CD3δ antibodies are used for T cell identification, diagnosis of immunodeficiencies, and exploration of T cell signaling mechanisms.

References:

1. Wei, Zhengyu et al. Bioengineered vol. 13,5 (2022): 13784-13800.
2. Yang, Yi et al. Immunotherapy vol. 12,1 (2020): 25-35.
3. Xin, Weizhi et al. Nature vol. 630,8015 (2024): 222-229.