Recombinant Mouse EMR1/ADGRE1/(F4/80) protein (His Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg0788
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse EMR1/ADGRE1/(F4/80) protein Gln28-Glu644 (Accession# Q61549) with a His tag at the C-terminus. |
| GeneID | 13733 |
| Accession | Q61549 |
| PredictedSize | 67.7 kDa |
| SDS-PAGE | kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.
References:
1.J M Austyn, et al. (1981) Eur J Immunol. 11(10):805-15. 2.A J McKnight, et al. (1996) J Biol Chem. 271(1):486-9. 3.I D Haidl, et al. (1996) Eur J Immunol. 26(5):1139-46. 4.Siamon Gordon, et al. (2011) Eur J Immunol. 41(9):2472-6.