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Recombinant Mouse EpCAM/CD326 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1883

Validation Data Gallery

  • Purity of Recombinant Mouse EpCAM was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse EpCAM was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse EpCAM protein Gln24-Thr266 (Accession# Q99JW5) with a His tag at the C-terminus.
GeneID 17075
Accession Q99JW5
PredictedSize 28.7 kDa
SDS-PAGE 31-36 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation.

References:

1. Bernardina T F van der Gun, et al. (2010) Carcinogenesis. Nov;31(11):1913-21.
2. Graham Carpenter, Monica Red Brewer. (2009) Cancer Cell. Mar 3;15(3):165-6.
3. Carlo Patriarca, et al. (2012) Cancer Treat Rev. Feb;38(1):68-75.
4. Sannia Imrich, et al. (2012) Cell Adh Migr. Jan-Feb;6(1):30-8.
5. Christof Pauli, et al. (2003) Cancer Lett. Apr 10;193(1):25-32.