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Mouse IFNAR2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.156-10 ng/mL

Sandwich ELISA: 39.1-2500 pg/mL

Cat no : MP03193-1

Synonyms

IFN alpha REC, IFN alpha/beta receptor 2, IFN R, IFN R 2, IFNABR

Validation Data Gallery

  • Sandwich ELISA standard curve of MP03193-1, Mouse IFNAR2 Recombinant Matched Antibody Pair - PBS only. 87764-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6269. 87764-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

    Sandwich ELISA standard curve of MP03193-1

    Sandwich ELISA standard curve of MP03193-1, Mouse IFNAR2 Recombinant Matched Antibody Pair - PBS only. 87764-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6269. 87764-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • The mean IFNAR2 concentration was determined to be 44.7 ng/mL in mouse liver extract based on a 8.4 mg/mL extract load, 13.1 ng/mL in mouse brain extract based on a 6.3 mg/mL extract load and 6.2 ng/mL in mouse colon extract based on a 4.9 mg/mL extract load.

    Sandwich ELISA sample test of MP03193-1

    The mean IFNAR2 concentration was determined to be 44.7 ng/mL in mouse liver extract based on a 8.4 mg/mL extract load, 13.1 ng/mL in mouse brain extract based on a 6.3 mg/mL extract load and 6.2 ng/mL in mouse colon extract based on a 4.9 mg/mL extract load.

  • Cytometric bead array standard curve of MP03193-1, MOUSE IFNAR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87764-2-PBS. Detection antibody: 87764-1-PBS. Standard: Eg6269. Range: 0.156-10 ng/mL.

    Cytometric bead array standard curve of MP03193-1

    Cytometric bead array standard curve of MP03193-1, MOUSE IFNAR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87764-2-PBS. Detection antibody: 87764-1-PBS. Standard: Eg6269. Range: 0.156-10 ng/mL.

  • The mean IFNAR2 concentration was determined to be 56.7 ng/mL in mouse liver tissue extract based on a 8.4 mg/mL extract load, 11.3 ng/mL in mouse brain tissue extract based on a 6.3 mg/mL extract load.

    Cytometric bead array sample test of MP03193-1

    The mean IFNAR2 concentration was determined to be 56.7 ng/mL in mouse liver tissue extract based on a 8.4 mg/mL extract load, 11.3 ng/mL in mouse brain tissue extract based on a 6.3 mg/mL extract load.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP03193-1 targets IFNAR2 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.156-10 ng/mL (Cytometric Bead Array); 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: Mouse IFNAR2 Recombinant monoclonal antibody, PBS Only (Capture) 87764-2-PBS (252155C12). 100 μg. Concentration 1 mg/ml.

Detection antibody: Mouse Interferon alpha/beta receptor 2 Recombinant monoclonal antibody, PBS Only (Detector) 87764-1-PBS (252155E7). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Eg6269

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 87764-2-PBS
Tested Reactivity mouse
Immunogen IFNAR2 fusion protein Eg6269
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interferon (alpha and beta) receptor 2
Synonyms IFN-alpha/beta receptor 2, IFN-R-2, Interferon alpha/beta receptor 2, Type I interferon receptor 2
GenBank accession numberNM_010509
Gene symbol Ifnar2
UniprotO35664-1
Gene ID (NCBI) 15976
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 87764-1-PBS
Tested Reactivity mouse
Immunogen Interferon alpha/beta receptor 2 fusion protein Eg6269
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interferon (alpha and beta) receptor 2
Synonyms
GenBank accession numberNM_010509
Gene symbol Ifnar2
UniprotO35664-1
Gene ID (NCBI) 15976
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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