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Recombinant Mouse IL-12B/IL-12 p40 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0299

Validation Data Gallery

  • Purity of Recombinant Mouse IL-12B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse IL-12B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse IL-12B protein Met23-Ser335 (Accession# P43432) with a His tag at the C-terminus.
GeneID 16160
Accession P43432
PredictedSize 36.6 kDa
SDS-PAGE 40-55 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-12p40 is known as a component of the bioactive cytokines interleukin IL-12 and IL-23, which can exist in a monomeric or homodimeric form. IL-12p40 is produced primarily by activated inflammatory cells including macrophages, neutrophils, microglia and dendritic cells (DCs). The most widely appreciated function of IL-12p40 is to provide a negative feedback loop by competitively binding to the IL-12 receptor. Additionally, IL-12p40 can also act as a chemoattractant for macrophages and promote the migration of bacterially stimulated dendritic cells. It is also associated with several pathogenic inflammatory responses such as silicosis, graft rejection.

References:

1. Cooper AM. et al. (2007). Trends Immunol. 28:33-8.
2. Wang SZ. et al. (2004). J Immunol. 173:4040-9.
3. Bielekova B. et al. (2012). PLoS One. 7:e48370.
4. Xu J. et al. (2016). Cell Death Differ. 23:1941-1951.