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Recombinant Mouse MKI67 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

N-rFc

Activity

not tested

Cat no : Eg1548

Validation Data Gallery

  • Purity of Recombinant Mouse MKI67 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse MKI67 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse MKI67 protein Asp1816-Pro2163 (Accession# E9PVX6-1) with a rabbit IgG Fc tag at the N-terminus
GeneID 17345
Accession E9PVX6-1
PredictedSize 68.5 kDa
SDS-PAGE 85-100 kDa, reducing (R) condition
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

MKI67 gene encodes a nuclear protein, antigen KI-67, which was identified by monoclonal antibody Ki-67. Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. The antigen Ki-67 is a human nuclear protein the expression of which is strictly associated with cell proliferation and which is widely used in routine pathology as a "proliferation marker" to measure the growth fraction of cells in human tumors. During mitosis, Ki-67 is essential for formation of the perichromosomal layer (PCL), a ribonucleoprotein sheath coating the condensed chromosomes. Ki-67 acts to prevent aggregation of mitotic chromosomes.

References:

1.Schlüter C, et al. (1993) J Cell Biol. 123(3):513-22.
2.Sun X, Kaufman PD. (2018) Chromosoma. 127(2):175-186.