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Recombinant Mouse Ki67/MKI67 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1548

Validation Data Gallery

  • Purity of Recombinant Mouse MKI67 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse MKI67 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse MKI67 protein Asp1816-Pro2163 (Accession# E9PVX6-1) with a rabbit IgG Fc tag at the N-terminus
GeneID 17345
Accession E9PVX6-1
PredictedSize 65.5 kDa
SDS-PAGE 85-100 kDa, reducing (R) condition
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Ki67 is a nuclear protein encoded by the MKI67 gene, widely recognized as a marker for cell proliferation. It is expressed in all active phases of the cell cycle (G1, S, G2, and M) but absent in quiescent cells (G0). Initially identified by the monoclonal antibody Ki-67, its expression correlates with the growth fraction of tumor cells, making it a critical tool in oncology for assessing tumor aggressiveness and patient prognosis. Beyond proliferation, recent studies highlight Ki67's role in chromatin organization, ribosomal RNA transcription, and mitotic chromosome architecture, underscoring its multifunctionality in cellular processes.

References:

1. Gerdes, J et al.Journal of immunology (Baltimore, Md. : 1950) vol. 133,4 (1984): 1710-5.
2. Luporsi, Elisabeth et al. Breast cancer research and treatment vol. 132,3 (2012): 895-915.
3. Scholzen, T, and J Gerdes. Journal of cellular physiology vol. 182,3 (2000): 311-22.
4. Aung, Thazin Nwe et al. Mod Pathol. Inc vol. 34,7 (2021): 1261-1270.