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Recombinant mouse Ly6g protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

C-rFc

Activity

not tested

Cat no : Eg2609

Validation Data Gallery

  • Purity of Recombinant Mouse Ly6g was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Ly6g was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse Ly6g protein Ala21-Ser114 (Accession# P35461) with a rabbit IgG Fc tag at the C-terminus
GeneID 546644
Accession P35461
PredictedSize 36.0 kDa
SDS-PAGE 35-45 kDa, reducing (R) condition
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow. The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen. Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils . The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C.

References:

1. T J Fleming, et al. (1993) J Immunol. 151(5):2399-408.
2. E Lagasse, et al. (1996) J Immunol Methods. 197(1-2):139-50.
3. Pui Y Lee, et al. (2013) J Leukoc Biol. 94(4):585-94.