Recombinant Mouse Surfactant protein D protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2924

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Validation Data Gallery

• 

  Purity of Recombinant Mouse Surfactant protein D was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse Surfactant protein D protein Ala20-Phe374 (Accession# P50404) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 20390
• Accession: P50404
• PredictedSize: 61.7 kDa
• SDS-PAGE: 67-75 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Surfactant Protein D (SP‐D) is a pattern recognition molecule that belongs to the collectin family expressed in multiple human organ systems, including alveolar type II cells and Clara cells. The surfactant protein D‐encoding gene (SFTPD) is located at the genomic position 10q22.2‐23.1. SP‐D plays an important role in the innate immune system by binding bacteria, viruses, fungi, and parasites for clearance via opsonization in phagocytes, as well as aiding in the removal of allergens.

References:

1. Sorensen GL. (2018) Front Med. 5:18. 
2. Sorensen GL, et al. (2014) J Pediatr. 165(4):683‐689.
3. Wright JR. (2005) Nat Rev Immunol. 5(1):58‐68.