NCAM2 Monoclonal antibody

NCAM2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rabbit, Mouse, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D6C12

Cat no : 68205-1-Ig

Synonyms

N CAM 2, NCAM 2, NCAM2, NCAM21

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Validation Data Gallery

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  WB analysis using 68205-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68205-1-Ig (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 68205-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68205-1-Ig (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 68205-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCAM2 antibody (68205-1-Ig, Clone: 1D6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 68205-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCAM2 antibody (68205-1-Ig, Clone: 1D6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rabbit brain tissue, LNCaP cells, rat brain tissue, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68205-1-Ig targets NCAM2 in WB, IF, ELISA applications and shows reactivity with Human, Rabbit, Mouse, Rat samples.

• Tested Reactivity: Human, Rabbit, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NCAM2 fusion protein Ag4935
• Full Name: neural cell adhesion molecule 2
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 93 kDa, 125 kDa
• GenBank Accession Number: BC052946
• Gene Symbol: NCAM2
• Gene ID (NCBI): 4685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.