NCAPH Monoclonal antibody, PBS Only

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  WB analysis using 67655-1-Ig (same clone as 67655-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67655-1-Ig (NCAPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67655-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67655-1-Ig (same clone as 67655-1-PBS)

  WB result of NCAPH antibody (67655-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPH transfected HEK-293 cells. This data was developed using the same antibody clone with 67655-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67655-1-Ig (same clone as 67655-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67655-1-Ig (NCAPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67655-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67655-1-Ig (same clone as 67655-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67655-1-Ig (NCAPH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67655-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67655-1-Ig (same clone as 67655-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67655-1-Ig (NCAPH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67655-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67655-1-Ig (same clone as 67655-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using NCAPH antibody (67655-1-Ig, Clone: 3D2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67655-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NCAPH Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67655-1-PBS

Clone No.3D2F11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

3D2F11, BRRN, BRRN1, CAP H, CAPH

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Product Information

67655-1-PBS targets NCAPH in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27748

  Product name: Recombinant human NCAPH protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 392-741 aa of BC024211

  Sequence: SWKEPCQVQSCQEEMISLGDGDIRTMCPLLSMKPGEYSYFSPRTMSMWAGPDHWRFRPRRKQDAPSQSENKKKSTKKDFEIDFEDDIDFDVYFRKTKAATILTKSTLENQNWRATTLPTDFNYNVDTLVQLHLKPGTRLLKMAQGHRAETEHYEEIEDYDYNNPNDTSNFCPGLQAADSDDEDLDDLFVGPVGNSDLSPYPCHPPKTAQQNGDTPEAQGLDITTYGESNLVAEPQKVNKIEIHYAKTAKKMDMKKLKQSMWSLLTALSGKEADAEANHREAGKEAALAEVADEKMLSGLTKDLQRSLPPVMAQNLSIPLAFACLLHLANEKNLKLEGTEDLSDVLVRQGD

• Full Name: non-SMC condensin I complex, subunit H
• Calculated Molecular Weight: 741 aa, 83 kDa
• Observed Molecular Weight: 83-100 kDa
• GenBank Accession Number: BC024211
• Gene Symbol: NCAPH
• Gene ID (NCBI): 23397
• RRID: AB_2918495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15003
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Non-SMC condensin I complex subunit H (NCAPH) is one of the three non-SMC subunits in condensin I, which belongs to a recently defined superfamily of proteins termed kleisins. Another two non-SMC subunits, CAP-D2 and CAP-G, share a highly degenerate repeating motif known as HEAT repeat. Some studies show that each subunit is essential for viability and plays an important role in mitotic chromosome architecture and segregation. In recent years, researchers found that the high expression of NCAPH was associated with poor prognosis in patients with non-small cell lung cancer and prostate cancer. Downregulation of NCAPH inhibited the proliferation, migration, and invasion of several cancer cells significantly. Moreover, NCAPH was involved in the regulation of mature chromosome condensation and DNA damage. These data suggest that NCAPH may be a key carcinogen involved in the development and progression of human malignant tumors. ( PMID: 28300828, PMID: 33311486 )