NCAPH Monoclonal antibody

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• KD/KO Validated

NCAPH Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3D2F11

Cat no : 67655-1-Ig

Synonyms

Barren homolog protein 1, BRRN, BRRN1, CAP H, CAPH, Condensin complex subunit 2, HCAP H, KIAA0074, NCAPH, XCAP H homolog

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Validation Data Gallery

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  WB analysis using 67655-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67655-1-Ig (NCAPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HEK-293 using 67655-1-Ig

  WB result of NCAPH antibody (67655-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPH transfected HEK-293 cells.

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  WB analysis using 67655-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67655-1-Ig (NCAPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67655-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67655-1-Ig (NCAPH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67655-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67655-1-Ig (NCAPH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 67655-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using NCAPH antibody (67655-1-Ig, Clone: 3D2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, NIH/3T3 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67655-1-Ig targets NCAPH in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NCAPH fusion protein Ag27748
• Full Name: non-SMC condensin I complex, subunit H
• Calculated Molecular Weight: 741 aa, 83 kDa
• Observed Molecular Weight: 83-100 kDa
• GenBank Accession Number: BC024211
• Gene Symbol: NCAPH
• Gene ID (NCBI): 23397
• RRID: AB_2918495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Non-SMC condensin I complex subunit H (NCAPH) is one of the three non-SMC subunits in condensin I, which belongs to a recently defined superfamily of proteins termed kleisins. Another two non-SMC subunits, CAP-D2 and CAP-G, share a highly degenerate repeating motif known as HEAT repeat. Some studies show that each subunit is essential for viability and plays an important role in mitotic chromosome architecture and segregation. In recent years, researchers found that the high expression of NCAPH was associated with poor prognosis in patients with non-small cell lung cancer and prostate cancer. Downregulation of NCAPH inhibited the proliferation, migration, and invasion of several cancer cells significantly. Moreover, NCAPH was involved in the regulation of mature chromosome condensation and DNA damage. These data suggest that NCAPH may be a key carcinogen involved in the development and progression of human malignant tumors. ( PMID: 28300828, PMID: 33311486 )

Publications

Species	Application	Title
human	WB	Science; Defining the KRAS- and ERK-dependent transcriptome in KRAS-mutant cancers; Authors - Jeffrey A Klomp