CoraLite® Plus 488-conjugated NCL Polyclonal antibody

NCL Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG


human, mouse, rat


IF, FC (Intra)


CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10556


C23, FLJ45706, NCL, nucleolin, Protein C23

Tested Applications

Positive IF detected inHEK-293 cells
Positive FC detected inHeLa cells

Recommended dilution

Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL488-10556 targets NCL in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCL fusion protein Ag0859
Full Name nucleolin
Calculated molecular weight 76 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC006494
Gene symbol NCL
Gene ID (NCBI) 4691
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)


Product Specific Protocols
IF protocol for CL Plus 488 NCL antibody CL488-10556Download protocol
FC protocol for CL Plus 488 NCL antibody CL488-10556Download protocol
Standard Protocols
Click here to view our Standard Protocols