NCL Polyclonal antibody

NCL Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10556-1-AP

Synonyms

C23, NCL, nucleolin, Protein C23



Tested Applications

Positive WB detected inHEK-293 cells, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

10556-1-AP targets NCL in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCL fusion protein Ag0859
Full Name nucleolin
Calculated molecular weight 76 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC006494
Gene symbol NCL
Gene ID (NCBI) 4691
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)

Protocols

Product Specific Protocols
WB protocol for NCL antibody 10556-1-APDownload protocol
IHC protocol for NCL antibody 10556-1-APDownload protocol
IF protocol for NCL antibody 10556-1-APDownload protocol
IP protocol for NCL antibody 10556-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

C9orf72 nucleotide repeat structures initiate molecular cascades of disease.

Authors - Aaron R Haeusler
ratIF

Neuron

Antisense Proline-Arginine RAN Dipeptides Linked to C9ORF72-ALS/FTD Form Toxic Nuclear Aggregates that Initiate In Vitro and In Vivo Neuronal Death.

Authors - Xinmei Wen
mouseWB

Genome Res

The nuclear matrix protein HNRNPU maintains 3D genome architecture globally in mouse hepatocytes.

Authors - Hui Fan
mouseWB

Protein Cell

Nuclear m6A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos.

Authors - Chuan Chen
human,mouseIF

EMBO J

The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.

Authors - Ella Doron-Mandel
humanIF

Acta Neuropathol

Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.

Authors - Johnathan Cooper-Knock

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HEK293T for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293T