NDUFAF3 Polyclonal antibody, PBS Only

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  WB analysis using 25621-1-AP (same clone as 25621-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25621-1-AP (NDUFAF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 25621-1-AP (same clone as 25621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25621-1-AP (NDUFAF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 25621-1-AP (same clone as 25621-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFAF3 antibody (25621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

NDUFAF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 25621-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NDUF3, E3 3, C3orf60, 2P1

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Product Information

25621-1-PBS targets NDUFAF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22514

  Product name: Recombinant human NDUFAF3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-184 aa of BC002873

  Sequence: MATALALRSLYRARPSLRCPPVELPWAPRRGHRLSPADDELYQRTRISLLQREAAQAMYIDSYNSRGFMINGNRVLGPCALLPHSVVQWNVGSHQDITEDSFSLFWLLEPRIEIVVVGTGDRTERLQSQVLQAMRQRGIAVEVQDTPNACATFNFLCHEGRVTGAALIPPPGGTSLTSLGQAAQ

• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3
• Calculated Molecular Weight: 184 aa, 20 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC002873
• Gene Symbol: NDUFAF3
• Gene ID (NCBI): 25915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BU61
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NDUFAF3 has been assigned to be an assembly factor of the Q-module because it physically associates with Q-module subassemblies during complex I biogenesis (PMID: 27986404).