NDUFB4 Polyclonal antibody, PBS Only

• 

  WB analysis of mouse heart using 27931-1-AP (same clone as 27931-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 27931-1-AP (NDUFB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27931-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 27931-1-AP (same clone as 27931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27931-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 27931-1-AP (same clone as 27931-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFB4 antibody (27931-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27931-1-PBS in a different storage buffer formulation.

NDUFB4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 27931-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NADH-ubiquinone oxidoreductase B15 subunit, Complex I-B15, Complex I B15, CI-B15, CI B15

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Product Information

27931-1-PBS targets NDUFB4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27484

  Product name: Recombinant human NDUFB4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-128 aa of BC000855

  Sequence: MSFPKYKPSSLRTLPETLDPAEYNISPETRRAQAERLAIRAQLKREYLLQYNDPNRRGLIENPALLRWAYARTINVYPNFRPTPKNSLMGALCGFGPLIFIYYIIKTERDRKEKLIQEGKLDRTFHLS

• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC000855
• Gene Symbol: NDUFB4
• Gene ID (NCBI): 4710
• RRID: AB_2881012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95168
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.