NDUFB4 Polyclonal antibody

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• NDUFB4 Polyclonal antibody (27931-1-AP)

NDUFB4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27931-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

B15, CI B15, CI-B15, Complex I B15, Complex I-B15

• 

  WB analysis of mouse heart using 27931-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 27931-1-AP (NDUFB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 27931-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 27931-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 27931-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse stomach using 27931-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat cerebellum using 27931-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat stomach using 27931-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 27931-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFB4 antibody (27931-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: human stomach cancer tissue, mouse stomach tissue, rat cerebellum tissue, mouse skeletal muscle tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27931-1-AP targets NDUFB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27484

  Product name: Recombinant human NDUFB4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-128 aa of BC000855

  Sequence: MSFPKYKPSSLRTLPETLDPAEYNISPETRRAQAERLAIRAQLKREYLLQYNDPNRRGLIENPALLRWAYARTINVYPNFRPTPKNSLMGALCGFGPLIFIYYIIKTERDRKEKLIQEGKLDRTFHLS

• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC000855
• Gene Symbol: NDUFB4
• Gene ID (NCBI): 4710
• RRID: AB_2881012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95168
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The NDUFB4 Polyclonal antibody (Cat# 27931-1-AP) has 2 total citations. These appear in Cells and Investigative Ophthalmology & Visual Science. The cited research covers ovarian cancer cell apoptosis and ferroptosis under energy stress conditions, as well as comparative proteomic profiling of uveal melanoma subtypes. Both studies utilized the antibody for Western blot analysis in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cells; Metformin Induces Apoptosis and Ferroptosis of Ovarian Cancer Cells Under Energy Stress Conditions; Authors - Yulun Wu
human	WB	Invest Ophthalmol Vis Sci; Comparative Proteomic Profiling of Microdissected Spindle and Epithelioid Uveal Melanoma Subtypes.; Authors - Yu Luan