NEBL Polyclonal antibody, PBS Only

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  WB analysis using 21497-1-AP (same clone as 21497-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21497-1-AP (NEBL antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21497-1-AP (NEBL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21497-1-AP (NEBL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using NEBL antibody (21497-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

NEBL Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 21497-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Nebulette, LNEBL, C10orf113, Actin-binding Z-disk protein, Actin binding Z disk protein

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Product Information

21497-1-PBS targets NEBL in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEBL fusion protein Ag15882
• Full Name: nebulette
• Calculated Molecular Weight: 1014 aa, 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC110453
• Gene Symbol: NEBL
• Gene ID (NCBI): 10529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O76041
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nebulette, also known as NEBL, is a cardiac-specific isoform belonging to the nebulin family of proteins. Nebulette has 23 modular repeat structures of 35 amino acid residues each. Nebulette has an acidic region with unknown structure at its N-terminus, and a serine-rich region adjacent to an SH3 domain at its C-terminus (PMID: 2037050, 20951588). Nebulette functionally links sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres (PubMed:27733623). Nebulette is abundantly expressed in cardiac muscle at Z-disc regions, but not in skeletal or smooth muscle.