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NEU3 Monoclonal antibody

NEU3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C9C5

Cat no : 67098-1-Ig

Synonyms

cDNA FLJ78026, FLJ12388, NEU3, NEU3 protein, SIAL3



Tested Applications

Positive WB detected inPC-3 cells, COLO 320 cells, HT-29 cells, K-562 cells, THP-1 cells, Caco-2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67098-1-Ig targets NEU3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NEU3 fusion protein Ag27272
Full Name sialidase 3 (membrane sialidase)
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberNM_006656.5
Gene Symbol NEU3
Gene ID (NCBI) 10825
RRIDAB_2882403
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NEU3 antibody 67098-1-IgDownload protocol
IHC protocol for NEU3 antibody 67098-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols