NHE3 Polyclonal antibody

NHE3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 27190-1-AP

Synonyms

SLC9A3, NHE-3

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Validation Data Gallery

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  WB analysis of mouse small intestine using 27190-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 27190-1-AP (NHE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 27190-1-AP

  IP result of anti-NHE3 (IP:27190-1-AP, 4ug; Detection:27190-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse kidney using 27190-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH9). Arrows show brush border.

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  IHC staining of mouse kidney using 27190-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse small intestine tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

27190-1-AP targets NHE3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NHE3 fusion protein Ag25637
• Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3
• Calculated Molecular Weight: 834 aa, 93 kDa
• Observed Molecular Weight: 80-100 kDa
• GenBank Accession Number: BC101669
• Gene Symbol: NHE3
• Gene ID (NCBI): 6550
• RRID: AB_2880793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC9A3, also known as NHE3, belongs to the sodium hydrogen exchange protein family (NHE) and is mainly responsible for regulating the concentration of sodium and hydrogen ions inside and outside the cell. SLC9A3 is a Major apical Na+/H+ exchanger in kidney and intestine, playing an important role in renal and intestine Na+ absorption and blood pressure regulation (PMID:24622516, 2635877). Mutations in SLC9A3 cause Congenital Sodium Diarrhea (PMID: 26358773, 31276831).

Publications

Species	Application	Title
mouse	WB	Hypertension; Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.; Authors - Shuai Shao
mouse	WB	Hypertension; Podocyte Injury Augments Intrarenal Angiotensin II Generation and Sodium Retention in a Megalin-Dependent Manner.; Authors - Masahiro Koizumi
pig	WB	J Virol; Pemetrexed alleviates piglet diarrhea by blocking the interaction between porcine epidemic diarrhea virus nucleocapsid protein and Ezrin; Authors - Shujuan Zhang
mouse	WB	Food Funct; EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.; Authors - Jian Fang
pig	IHC	Front Vet Sci; Effects of Guava (Psidium guajava L.) Leaf Extract on the Metabolomics of Serum and Feces in Weaned Piglets Challenged by Escherichia coli.; Authors - Dingfa Wang
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen