CoraLite® Plus 488-conjugated NIT2 Monoclonal antibody

NIT2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E5B1

Cat no : CL488-68169

Synonyms

NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2

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Validation Data Gallery

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  IF Staining of U-251 using CL488-68169

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 NIT2 antibody (CL488-68169, Clone: 1E5B1 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: U-251 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68169 targets NIT2 in IF applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NIT2 fusion protein Ag9889
• Full Name: nitrilase family, member 2
• Calculated Molecular Weight: 276 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC020620
• Gene Symbol: NIT2
• Gene ID (NCBI): 56954
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.