NR1D1 Polyclonal antibody, PBS Only

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  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)

  WB result of anti-NR1D1 (14506-1-AP,1:1000) with A549 cell by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)

  WB result of anti-NR1D1 (14506-1-AP,1:500) with A549 treated with Cycloheximide by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 14506-1-AP (same clone as 14506-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 14506-1-AP (same clone as 14506-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 14506-1-AP (same clone as 14506-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 14506-1-AP (NR1D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NR1D1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14506-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ear 1, EAR1, EAR-1, HREV, Rev erbA alpha

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Product Information

14506-1-PBS targets NR1D1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5964

  Product name: Recombinant human NR1D1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC056148

  Sequence: MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQR

• Full Name: nuclear receptor subfamily 1, group D, member 1
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC056148
• Gene Symbol: NR1D1
• Gene ID (NCBI): 9572
• RRID: AB_11182604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20393
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.