NR1H4 Recombinant antibody

NR1H4 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4B4

Cat no : 81820-2-RR

Synonyms

BAR, Bile acid receptor, Farnesoid X activated receptor, Farnesol receptor HRR 1, FXR, HRR 1, HRR1, NR1H4, RIP14, RXR interacting protein 14

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Validation Data Gallery

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  WB analysis using 81820-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81820-2-RR (NR1H4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MKN-45 cells, HepG2 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81820-2-RR targets NR1H4 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NR1H4 fusion protein Ag21948
• Full Name: nuclear receptor subfamily 1, group H, member 4
• Calculated Molecular Weight: 486 aa, 56 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC130573
• Gene Symbol: NR1H4
• Gene ID (NCBI): 9971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear Receptor subfamily 1, group H, member 4 (NR1H4, also known as FXR) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. NR1H4 is highly expressed in liver and has a role in lipid metabolism, whereas none of the other protein-coding genes in the region has known biological links with cholesterol, further supporting a role for NR1H4 in regulation of cholesterol levels. NR1H4 have four isoforms, i.e., FXRalpha1, FXRalpha2, FXRbeta1, and FXRbeta2, and each isoform has a different expressional level and transcriptional activity depending on its location within specific tissues. (PMID: 14733360, PMID: 30787420)