NRF1/nuclear respiratory factor 1 Monoclonal antibody

NRF1/nuclear respiratory factor 1 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b


Human, mouse, rat and More (1)







Cat no : 66832-1-Ig


ALPHA PAL, NRF 1, NRF1, nuclear respiratory factor 1

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

66832-1-Ig targets NRF1/nuclear respiratory factor 1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag28149
Full Name nuclear respiratory factor 1
Calculated Molecular Weight 522 aa, 56 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC016925
Gene Symbol NRF1
Gene ID (NCBI) 4899
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.


Product Specific Protocols
WB protocol for NRF1/nuclear respiratory factor 1 antibody 66832-1-IgDownload protocol
IHC protocol for NRF1/nuclear respiratory factor 1 antibody 66832-1-IgDownload protocol
IF protocol for NRF1/nuclear respiratory factor 1 antibody 66832-1-IgDownload protocol
FC protocol for NRF1/nuclear respiratory factor 1 antibody 66832-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Clin Transl Hepatol

Icaritin Attenuates Lipid Accumulation by Increasing Energy Expenditure and Autophagy Regulated by Phosphorylating AMPK.

Authors - Yue Wu

PLoS One

Supplementation with dietary omega-3 PUFA mitigates fetal brain inflammation and mitochondrial damage caused by high doses of sodium nitrite in maternal rats.

Authors - Jingchi Sun

Nitric Oxide

Hydrogen sulfide alleviates heart failure with preserved ejection fraction in mice by targeting mitochondrial abnormalities via PGC-1α

Authors - Shuying Huang

J Pharm Pharmacol

Styrax (Liquidambar orientalis Mill.) promotes mitochondrial function and reduces cardiac damage following myocardial ischemic injury: the role of the AMPK-PGC1α signaling pathway

Authors - Fei Mu

Folia Biol (Praha)

NRF1 Alleviated Oxidative Stress of Glioblastoma Cells by Regulating NOR1

Authors - Jiali Wang