CoraLite®488-conjugated NRF1/nuclear respiratory factor 1 Monoclonal antibody

NRF1/nuclear respiratory factor 1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

3C2B8

Cat no : CL488-66832

Synonyms

ALPHA PAL, NRF 1, NRF1, nuclear respiratory factor 1



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66832-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66832 targets NRF1/nuclear respiratory factor 1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag28149
Full Name nuclear respiratory factor 1
Calculated molecular weight 522 aa, 56 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC016925
Gene symbol NRF1
Gene ID (NCBI) 4899
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Protocols

Product Specific Protocols
IF protocol for CL488 NRF1/nuclear respiratory factor 1 antibody CL488-66832Download protocol
FC protocol for CL488 NRF1/nuclear respiratory factor 1 antibody CL488-66832Download protocol
Standard Protocols
Click here to view our Standard Protocols