CoraLite® Plus 488-conjugated NRF1/nuclear respiratory factor 1 Monoclonal antibody
NRF1/nuclear respiratory factor 1 Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3C2B8
Cat no : CL488-66832
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-66832 targets NRF1/nuclear respiratory factor 1 in IF, FC (Intra) applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | NRF1/nuclear respiratory factor 1 fusion protein Ag28149 |
Full Name | nuclear respiratory factor 1 |
Calculated molecular weight | 522 aa, 56 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC016925 |
Gene symbol | NRF1 |
Gene ID (NCBI) | 4899 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 NRF1/nuclear respiratory factor 1 antibody CL488-66832 | Download protocol |
FC protocol for CL Plus 488 NRF1/nuclear respiratory factor 1 antibody CL488-66832 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |