CoraLite® Plus 488-conjugated NRF1/nuclear respiratory factor 1 Monoclonal antibody

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  IF Staining of HepG2 using CL488-66832

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NRF1 antibody (CL488-66832, Clone: 3C2B8 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-66832

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NRF1/nuclear respiratory factor 1 (CL488-66832, Clone:3C2B8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NRF1/nuclear respiratory factor 1 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-66832

Clone No.3C2B8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66832 targets NRF1/nuclear respiratory factor 1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28149

  Product name: Recombinant human NRF1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 213-522 aa of BC016925

  Sequence: LKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLFMADRAGRKWILTDKATGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ

• Full Name: nuclear respiratory factor 1
• Calculated Molecular Weight: 522 aa, 56 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC016925
• Gene Symbol: NRF1
• Gene ID (NCBI): 4899
• RRID: AB_2883385
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16656
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.