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CoraLite®594-conjugated NRF1 Monoclonal antibody

NRF1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C2B8

Cat no : CL594-66832

Synonyms

ALPHA PAL, NRF 1, NRF1, nuclear respiratory factor 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated NRF1 Monoclonal antibody (CL594-66832)

    IF Staining of HepG2 using CL594-66832

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66832 (NRF1 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated NRF1 Monoclonal antibody (CL594-66832)

    FC experiment of HepG2 using CL594-66832

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NRF1 (CL594-66832, Clone:3C2B8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated NRF1 Monoclonal antibody (CL594-66832)

    FC experiment of HepG2 using CL594-66832

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NRF1 (CL594-66832, Clone:3C2B8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66832 targets NRF1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NRF1 fusion protein Ag28149
Full Name nuclear respiratory factor 1
Calculated Molecular Weight 522 aa, 56 kDa
GenBank Accession NumberBC016925
Gene Symbol NRF1
Gene ID (NCBI) 4899
RRIDAB_2883629
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 NRF1 antibody CL594-66832Download protocol
Standard Protocols
Click here to view our Standard Protocols