TrkA Monoclonal antibody

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  WB analysis using 60548-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60548-1-Ig (NTRK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NK-92 using 60548-1-Ig

  NK-92 cells were subjected to SDS PAGE followed by western blot with 60548-1-Ig (NTRK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60548-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60548-1-Ig (NTRK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

TrkA Monoclonal Antibody for WB, ELISA

Cat No. 60548-1-Ig

Clone No.3B11C7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

NTRK1, Trk A, TRK, p140 TrkA, MTC

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Tested Applications

• Positive WB detected in: Karpas-299 cells, NK-92 cells, SH-SY5Y cells, IMR-32 cells, TF-1 cells, K-562 cells, MOLT-4 cells, SK-N-SH cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60548-1-Ig targets TrkA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27985

  Product name: Recombinant human NTRK1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 80-277 aa of NM_001007792

  Sequence: MQHLELRDLRGLGELRNLTIVKSGLRFVAPDAFHFTPRLSRLNLSFNALESLSWKTVQGLSLQELVLSGNPLHCSCALRWLQRWEEEGLGGVPEQKLQCHGQGPLAHMPNASCGVPTLKVQVPNASVDVGDDVLLRCQVEGRGLEQAGWILTELEQSATVMKSGGLPSLGLTLANVTSDLNRKNVTCWAENDVGRAEVS

• Full Name: neurotrophic tyrosine kinase, receptor, type 1
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_001007792
• Gene Symbol: NTRK1
• Gene ID (NCBI): 4914
• RRID: AB_3670266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P04629
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NTRK1(Neurotrophic tyrosine kinase receptor 1) is located on chromosome 1q23.1 and is responsible for a rare genetic disorder, CIPA (congenital insensitivity to pain with anhidrosis) (PMID: 37907480). As a neurotrophin receptor, NTRK1 is abundantly expressed in human neuronal tissues and plays an integral role in the development and function of the human nervous system (PMID: 27843590). NTRK1 also regulates the survival and proliferation of cholinergic neurons (PMID: 20054812).