• Featured Product
  • KD/KO Validated

NUB1 Polyclonal antibody

NUB1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14343-1-AP


BS4, NEDD8 ultimate buster 1, NUB1, NUB1L, NYREN18

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Raji cells
Positive IP detected inHEK-293 cells
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14343-1-AP targets NUB1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUB1 fusion protein Ag5668
Full Name negative regulator of ubiquitin-like proteins 1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC046354
Gene Symbol NUB1
Gene ID (NCBI) 51667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteasome-associated protein NEDD8 ultimate buster 1 (NUB1) is an interferon-inducible protein that down-regulates NEDD8 expression and its conjugation system. Over-expression of NUB1 induces a growth-inhibitory effect in cells, and it mediates anti-proliferative actions and apoptosis in renal cell carcinoma cells through cell-cycle regulation. Expression of NUB1 leads to decreased modification of p53 with NEDD8 and stimulation of p53 ubiquitination. Recent finding revealed that NUB1, by regulating GSK3β levels, modulates tau phosphorylation and aggregation, and is a key player in neurodegeneration associated with tau pathology.


Product Specific Protocols
WB protocol for NUB1 antibody 14343-1-APDownload protocol
IF protocol for NUB1 antibody 14343-1-APDownload protocol
IP protocol for NUB1 antibody 14343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Biophys Res Commun

The role of NUB1 in α-synuclein degradation in Lewy body disease model mice.

Authors - Kunikazu Tanji


Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.

Authors - Fumiaki Mori

Brain Pathol

Phosphorylated NUB1 distinguishes α-synuclein in Lewy bodies from that in glial cytoplasmic inclusions in multiple system atrophy

Authors - Kunikazu Tanji