NUDC Polyclonal antibody

• Featured Product
• KD/KO Validated

NUDC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10681-1-AP

Synonyms

MNUDC, HNUDC

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Validation Data Gallery

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  WB analysis of HepG2 using 10681-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10681-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10681-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10681-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10681-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10681-1-AP

  IP result of anti-NUDC (IP:10681-1-AP, 4ug; Detection:10681-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human pancreas cancer using 10681-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10681-1-AP (NUDC antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 10681-1-AP

  Immunofluorescent analysis of HeLa cells using 10681-1-AP (NUDC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10681-1-AP

  Immunofluorescent analysis of HepG2 cells using 10681-1-AP (NUDC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10681-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10681-1-AP (NUDC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, HeLa cells, K-562 cells, SH-SY5Y cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HEK-293 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

10681-1-AP targets NUDC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDC fusion protein Ag0998
• Full Name: nuclear distribution gene C homolog (A. nidulans)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC006147
• Gene Symbol: NUDC
• Gene ID (NCBI): 10726
• RRID: AB_2282933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear distribution protein C (NudC) is a highly conserved dynein/dynactin associated protein implicated in mitosis and cytokinesis. NudC is localized on major mitotic structures, and is involved in regulating spindle formation stability of kinetochore microtubule attachments, and chromosomes congression in early mitosis. NudC also is required for multi-protein transport along axons in neurons.

Publications

Species	Application	Title
human	WB,IF	Cell Res; NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.; Authors - Cheng Zhang ; KD Validated
human	IF	Int J Mol Sci; N-Acetyl-D-Glucosamine Kinase Interacts with NudC and Lis1 in Dynein Motor Complex and Promotes Cell Migration.; Authors - Md Ariful Islam
human	WB	Mol Cell Biol; Substrate Trapping Proteomics Reveals Targets of the βTrCP2/FBXW11 Ubiquitin Ligase.; Authors - Tai Young Kim
human	WB	Front Cell Dev Biol; NudC L279P Mutation Destabilizes Filamin A by Inhibiting the Hsp90 Chaperoning Pathway and Suppresses Cell Migration.; Authors - Min Liu
mouse	WB,IHC	Reprod Domest Anim; Proteomic-based identification of oocyte maturation-related proteins in mouse germinal vesicle oocytes.; Authors - Senyang Cao