Validation Data Gallery
|Positive WB detected in
|NIH/3T3 cells, HEK-293 cells, HeLa cells, MCF-7 cells
|Positive IHC detected in
|human brain tissue, mouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HeLa cells, HepG2 cells
|Western Blot (WB)
|WB : 1:1000-1:6000
|IHC : 1:500-1:2000
|IF : 1:1000-1:4000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66335-1-Ig targets NUDT21 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Mouse / IgG1
|NUDT21 fusion protein Ag12260
|nudix (nucleoside diphosphate linked moiety X)-type motif 21
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein G purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.
A Cancer-Specific Ubiquitin Ligase Drives mRNA Alternative Polyadenylation by Ubiquitinating the mRNA 3' End Processing Complex.
LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation
J Mol Cell Biol
U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3' end processing core factors