NUDT21 Monoclonal antibody

NUDT21 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1C4C8

Cat no : 66335-1-Ig

Synonyms

CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, Nudix motif 21, NUDT21



Tested Applications

Positive WB detected inNIH/3T3 cells, HEK-293 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman brain tissue, mouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IPSee 1 publications below

Product Information

66335-1-Ig targets NUDT21 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NUDT21 fusion protein Ag12260
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 21
Calculated molecular weight 26 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001403
Gene symbol NUDT21
Gene ID (NCBI) 11051
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.

Protocols

Product Specific Protocols
WB protocol for NUDT21 antibody 66335-1-IgDownload protocol
IHC protocol for NUDT21 antibody 66335-1-IgDownload protocol
IF protocol for NUDT21 antibody 66335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Mol Cell

A Cancer-Specific Ubiquitin Ligase Drives mRNA Alternative Polyadenylation by Ubiquitinating the mRNA 3' End Processing Complex.

Authors - Seung Wook Yang