NUDT21 Monoclonal antibody, PBS Only

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  WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

  WB result of NUDT21 antibody (66335-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDT21 transfected NIH/3T3 cells. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66335-1-Ig (same clone as 66335-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66335-1-Ig (same clone as 66335-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NUDT21 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66335-1-PBS

Clone No.1C4C8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Nucleoside diphosphate-linked moiety X motif 21, CPSF5, CPSF25, Cleavage factor Im complex 25 kDa subunit, CFIm25

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Product Information

66335-1-PBS targets NUDT21 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12260

  Product name: Recombinant human NUDT21 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-222 aa of BC001403

  Sequence: MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRF

• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 21
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC001403
• Gene Symbol: NUDT21
• Gene ID (NCBI): 11051
• RRID: AB_2881715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O43809
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.