SLC13A3 Polyclonal antibody

SLC13A3 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26184-1-AP

Synonyms

hNaDC3, NaDC 3, NADC3, SDCT2, SLC13A3

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Validation Data Gallery

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  IP experiment of mouse kidney using 26184-1-AP

  IP result of anti-SLC13A3 (IP:26184-1-AP, 4ug; Detection:26184-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 26184-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (NaDC3 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26184-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (NaDC3 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 26184-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (SLC13A3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26184-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (SLC13A3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

26184-1-AP targets SLC13A3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC13A3 fusion protein Ag24182
• Full Name: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3
• Calculated Molecular Weight: 602 aa, 67 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC035966
• Gene Symbol: SLC13A3
• Gene ID (NCBI): 64849
• RRID: AB_2868533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC13A3, also named NADC3 and SDCT2, belongs to the SLC13A/DASS transporter (TC 2.A.47) family and NADC subfamily. SLC13A3 encodes the plasma membrane Na+ /Dicarboxylate Cotransporter 3 (NaDC3), which imports inside the cell four to six carbon dicarboxylates as well as N-acetylaspartate (NAA). SLC13A3 is mainly expressed in kidney, but also in brain, liver, placenta and eye (PMID: 30635937).

Publications

Species	Application	Title
rat	WB	Bioact Mater; An Injectable silk-based hydrogel as a novel biomineralization seedbed for critical-sized bone defect regeneration; Authors - Yuhui Zhu
human	WB,IF	Dev Cell; Itaconate uptake via SLC13A3 improves hepatic antibacterial innate immunity; Authors - Chao Chen ; KO Validated