NeuN Polyclonal antibody
NeuN Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF, Dot blot, ELISA
Conjugate
Unconjugated
Cat no : 26975-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, pig brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | rat cerebellum tissue, rat brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 5 publications below |
| IHC | See 8 publications below |
| IF | See 47 publications below |
Product Information
The immunogen of 26975-1-AP is NeuN Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NeuN fusion protein Ag25689 |
| Full Name | hexaribonucleotide binding protein 3 |
| Observed molecular weight | 46-52 kDa |
| GenBank accession number | NM_001082575 |
| Gene symbol | hCG_1776007 |
| Gene ID (NCBI) | 146713 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Function
NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).
Tissue specificity
NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).
Involvement in disease
· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).
Isoforms
There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).
Post-translational modifications
Currently not known.
Cellular localization
NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NeuN antibody 26975-1-AP | Download protocol |
| IHC protocol for NeuN antibody 26975-1-AP | Download protocol |
| IF protocol for NeuN antibody 26975-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Neurosci HDAC1 Silence Promotes Neuroprotective Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Mouse Model of Traumatic Brain Injury via PI3K/AKT Pathway. | ||
Mol Med Rep Neuroprotection of hydroxysafflor yellow A in experimental cerebral ischemia/reperfusion injury via metabolic inhibition of phenylalanine and mitochondrial biogenesis. | ||
Neuropathol Appl Neurobiol Expression of p16 and p21 in the frontal association cortex of ALS/MND brains suggest neuronal cell cycle dysregulation and astrocyte senescence in early stages of the disease. | ||
Stem Cells Int Combined Use of Chitosan and Olfactory Mucosa Mesenchymal Stem/Stromal Cells to Promote Peripheral Nerve Regeneration In Vivo. | ||
Oxid Med Cell Longev Photobiomodulation Therapy Ameliorates Glutamatergic Dysfunction in Mice with Chronic Unpredictable Mild Stress-Induced Depression. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH David (Verified Customer) (01-13-2020) | Good nuclear localisation, with strong signal and low background.
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FH Maxence (Verified Customer) (09-05-2019) | Labeling was neat without any background. Signal was quite strong and stable.
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FH Elena (Verified Customer) (07-31-2019) | Good antibody, stained in all cell lines we used it for.
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FH Kaspar (Verified Customer) (01-26-2018) |
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