NeuN Polyclonal antibody

NeuN Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF, Dot blot, ELISA

Conjugate

Unconjugated

Publications

183

Reviews (7)

Cat no : 26975-1-AP

Synonyms

FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3

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Validation Data Gallery

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  WB analysis using 26975-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 26975-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 26975-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 26975-1-AP

  pig brain tissue were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat cerebellum using 26975-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human gliomas using 26975-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26975-1-AP (NeuN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human gliomas using 26975-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26975-1-AP (NeuN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of rat cerebellum using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red).

• 

  IF Staining of rat cerebellum using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red).

• 

  IF Staining of mouse cerebellum using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:5000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 26975-1-AP (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66375-1-Ig (TUBB3 antibody, green).

• 

  IF Staining of rat cerebellum using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, pig brain tissue, rat brain tissue
• Positive IHC detected in: rat cerebellum tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: rat cerebellum tissue, rat brain tissue, mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:10000-1:40000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 28 publications below
• IHC: See 29 publications below
• IF: See 146 publications below

Product Information

26975-1-AP targets NeuN in WB, IHC, IF, Dot blot, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, zebrafish, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NeuN fusion protein Ag25689
• Full Name: hexaribonucleotide binding protein 3
• Observed molecular weight: 46-52 kDa
• GenBank accession number: NM_001082575
• Gene symbol: hCG_1776007
• Gene ID (NCBI): 146713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Function

NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).

Tissue specificity

NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).

Involvement in disease

· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).

Isoforms

There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).

Post-translational modifications

Currently not known.

Cellular localization

NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).

Publications

Species	Application	Title
mouse	IF	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse	IF	Sci Adv; CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.; Authors - Sefan Asamitsu
zebrafish	IHC	Nat Commun; An injury-induced serotonergic neuron subpopulation contributes to axon regrowth and function restoration after spinal cord injury in zebrafish.; Authors - Chun-Xiao Huang
mouse	IHC	Brain; Huntingtin silencing delays onset and slows progression of Huntington's disease: a biomarker study.; Authors - Hongshuai Liu
mouse	IHC	Front Behav Neurosci; Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in mice; Authors - Ying-Ke Jiang
mouse	WB,IF	Mater Today Bio; Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.; Authors - Luyu Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Monika (Verified Customer) (06-08-2023)

Very specific and strong signal. Used without antigen retrieval. Worked good for both NBT/BCIP-colorimetry and Alexa fluor-fluorescence detection.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse brain sections (Paraffin embedded)

FH

Russell (Verified Customer) (05-23-2023)

Antigen heat retrieval with Citrate buffer pH 6.0 at 100C for 20 min Permeabilized with 1% TX-100 Blocking Buffer PBST with 5% Donkey Serum

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Brain Organoid

FH

Irina (Verified Customer) (11-18-2021)

Good specific neuronal signal. Works nicely for double IF to look at co-localisation with other proteins.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: FFPE Human brain

FH

David (Verified Customer) (01-13-2020)

Good nuclear localisation, with strong signal and low background.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: SH-SY5Y

FH

Maxence (Verified Customer) (09-05-2019)

Labeling was neat without any background. Signal was quite strong and stable.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Brain

FH

Elena (Verified Customer) (07-31-2019)

Good antibody, stained in all cell lines we used it for.

• Applications: Western Blot,
• Cell Tissue Type: HeLA cells, hESCs

FH

Kaspar (Verified Customer) (01-26-2018)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: mouse