Validation Data Gallery
|Positive WB detected in||mouse brain tissue, pig brain tissue, rat brain tissue|
|Positive IHC detected in||rat cerebellum tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||rat cerebellum tissue, rat brain tissue, mouse cerebellum tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:10000-1:40000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
26975-1-AP targets NeuN in WB, IHC, IF, Dot blot, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat, zebrafish, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NeuN fusion protein Ag25689|
|Full Name||hexaribonucleotide binding protein 3|
|Observed molecular weight||46-52 kDa|
|GenBank accession number||NM_001082575|
|Gene ID (NCBI)||146713|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).
NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).
Involvement in disease
· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).
There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).
Currently not known.
NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).
Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.
CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.
An injury-induced serotonergic neuron subpopulation contributes to axon regrowth and function restoration after spinal cord injury in zebrafish.
Huntingtin silencing delays onset and slows progression of Huntington's disease: a biomarker study.
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Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in mice
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Monika (Verified Customer) (06-08-2023)
Very specific and strong signal. Used without antigen retrieval. Worked good for both NBT/BCIP-colorimetry and Alexa fluor-fluorescence detection.
Russell (Verified Customer) (05-23-2023)
Antigen heat retrieval with Citrate buffer pH 6.0 at 100C for 20 min Permeabilized with 1% TX-100 Blocking Buffer PBST with 5% Donkey Serum
Irina (Verified Customer) (11-18-2021)
Good specific neuronal signal. Works nicely for double IF to look at co-localisation with other proteins.
David (Verified Customer) (01-13-2020)
Good nuclear localisation, with strong signal and low background.
Maxence (Verified Customer) (09-05-2019)
Labeling was neat without any background. Signal was quite strong and stable.
Elena (Verified Customer) (07-31-2019)
Good antibody, stained in all cell lines we used it for.
Kaspar (Verified Customer) (01-26-2018)