NeuN Polyclonal antibody

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  WB analysis using 26975-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26975-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat cerebellum using 26975-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IF Staining of rat cerebellum using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red).

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  IF Staining of mouse cerebellum using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:5000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat cerebellum using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red).

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  IF Staining of rat brain using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 26975-1-AP (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66375-1-Ig (TUBB3 antibody, green).

• 

  IF Staining of rat cerebellum using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red).

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  IF Staining of mouse brain using 26975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NeuN antibody (26975-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  FC experiment of U-87 MG using 26975-1-AP

  1X10^6 U-87 MG cells were intracellularly stained with 0.2 ug Anti-Human NeuN (26975-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

NeuN Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Cat No. 26975-1-AP

Publications(305)

Reviews (10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (2)

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), Dot blot, ELISA

Synonyms

Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen, Neuronal nuclei antigen

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue
• Positive IHC detected in: rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
• Positive IF-Fro detected in: mouse brain tissue
• Positive FC (Intra) detected in: U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:10000-1:40000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 43 publications below
• IHC: See 47 publications below
• IF: See 245 publications below

Product Information

26975-1-AP targets NeuN in WB, IHC, IF-P, IF-Fro, FC (Intra), Dot blot, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, pig, monkey, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NeuN fusion protein Ag25689
• Full Name: hexaribonucleotide binding protein 3
• Observed Molecular Weight: 46-52 kDa
• GenBank Accession Number: NM_001082575
• Gene Symbol: NeuN
• Gene ID (NCBI): 146713
• RRID: AB_2880708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Function

NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).

Tissue specificity

NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).

Involvement in disease

· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).

Isoforms

There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).

Post-translational modifications

Currently not known.

Cellular localization

NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).

Publications

Species	Application	Title
mouse	IHC	Immunity; Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury; Authors - Yangning Lan
mouse	IF	Brain Behav Immun; Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease; Authors - Jiaxin Guan
mouse	IF	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse	IF	Sci Adv; CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.; Authors - Sefan Asamitsu
zebrafish	IHC	Nat Commun; An injury-induced serotonergic neuron subpopulation contributes to axon regrowth and function restoration after spinal cord injury in zebrafish.; Authors - Chun-Xiao Huang
mouse	IHC	Brain; Huntingtin silencing delays onset and slows progression of Huntington's disease: a biomarker study.; Authors - Hongshuai Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Raquel (Verified Customer) (07-12-2024)

Nice and clean staining. Immunofluorescence analysis in cryostat sections of 4% PFA fixed human brain organoid derived from iPSCs using 26975-1-AP NeuN antibody at dilution of 1:250 (under 20x lens). (Magenta: NeuN ; Blue: DAPI)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Human Brain organoids

NeuN Antibody Immunofluorescence validation (1:250 dilution) in Human Brain organoids (Cat no:26975-1-AP)

FH

Manohar (Verified Customer) (03-06-2024)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neuronal stem cells

FH

Damien (Verified Customer) (02-15-2024)

Works well in 2D staining of induced neurons, starting at 14days. Fixed and permeabilized with 3%BSA and 0.1% Triton

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Induced neurons

FH

Monika (Verified Customer) (06-08-2023)

Very specific and strong signal. Used without antigen retrieval. Worked good for both NBT/BCIP-colorimetry and Alexa fluor-fluorescence detection.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse brain sections (Paraffin embedded)

FH

Russell (Verified Customer) (05-23-2023)

Antigen heat retrieval with Citrate buffer pH 6.0 at 100C for 20 min Permeabilized with 1% TX-100 Blocking Buffer PBST with 5% Donkey Serum

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Brain Organoid

FH

Irina (Verified Customer) (11-18-2021)

Good specific neuronal signal. Works nicely for double IF to look at co-localisation with other proteins.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: FFPE Human brain

FH

David (Verified Customer) (01-13-2020)

Good nuclear localisation, with strong signal and low background.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: SH-SY5Y

FH

Maxence (Verified Customer) (09-05-2019)

Labeling was neat without any background. Signal was quite strong and stable.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Brain

FH

Elena (Verified Customer) (07-31-2019)

Good antibody, stained in all cell lines we used it for.

• Applications: Western Blot,
• Cell Tissue Type: HeLA cells, hESCs

FH

Kaspar (Verified Customer) (01-26-2018)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: mouse