PALMD Recombinant monoclonal antibody

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  WB analysis using 87300-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87300-3-RR (PALMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87300-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87300-3-RR (PALMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 87300-3-RR

  IP result of anti-PALMD (IP:87300-3-RR, 4ug; Detection:87300-3-RR 1:2000) with HeLa cells lysate 1320 ug.

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  Affinity of 87300-3-RR

  Biolayer interferometry (BLl) kinetic assays of 87300-3-RR against Human PALMD were performed. The affinity constant is 0.537 nM.

PALMD Uni-rAb^® Recombinant Antibody for WB, IP, ELISA

Cat No. 87300-3-RR

Clone No.252551B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

C1orf11, Palmdelphin, PALML, Paralemmin-like protein

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, L02 cells, HUVEC cells, rat lung tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87300-3-RR targets PALMD in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9796

  Product name: Recombinant human PALMD protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 218-551 aa of BC012570

  Sequence: YAVSSNHSAAYNGTDGLAPVEVEELLRQASERNSKSPTEYHEPVYANPFYRPTTPQRETVTPGPNFQERIKIKTNGLGIGVNESIHNMGNGLSEERGNNFNHISPIPPVPHPRSVIQQAEEKLHTPQKRLMTPWEESNVMQDKDAPSPKPRLSPRETIFGKSEHQNSSPTCQEDEEDVRYNIVHSLPPDINDTEPVTMIFMGYQQAEDSEEDKKFLTGYDGIIHAELVVIDDEEEEDEGEAEKPSYHPIAPHSQVYQPAKPTPLPRKRSEASPHENTNHKSPHKNSISLKEQEESLGSPVHHSPFDAQTTGDGTEDPSLTALRMRMAKLGKKVI

• Full Name: palmdelphin
• Calculated Molecular Weight: 551 aa, 63 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC012570
• Gene Symbol: PALMD
• Gene ID (NCBI): 54873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NP74
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Palmdelphin, encoded by PALMD gene, is a newly identified cytosolic isoform of paralemmin-1, a lipid raft-associated protein implicated in cell shape control. Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Previous finding suggest that palmdelphin may peripherally associate with endomembranes or cytoskeleton-linked structures. Palmdelphin appears to be 80 kDa in Western blotting, larger than the predicated of 63 kDa, and this may be due to the acidic nature of this protein or its posttranslational modification.