PANK2 Polyclonal antibody, PBS Only

• 

  WB analysis using 11001-1-AP (same clone as 11001-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11001-1-AP (same clone as 11001-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 11001-1-AP (same clone as 11001-1-PBS)

  IP result of anti-PANK2 (IP:11001-1-AP, 3ug; Detection:11001-1-AP 1:800) with mouse brain tissue lysate 9500ug. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11001-1-AP (same clone as 11001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11001-1-AP (PANK2 antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 11001-1-AP (same clone as 11001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 11001-1-AP (same clone as 11001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11001-1-AP (same clone as 11001-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PANK2 antibody (11001-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

PANK2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11001-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Pantothenate kinase 2, mitochondrial intermediate form, mPanK2, iPanK2, hPanK2, EC:2.7.1.33

---

Product Information

11001-1-PBS targets PANK2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1413

  Product name: Recombinant human PANK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 328-570 aa of BC009421

  Sequence: IGDLQLCKLDELDCLIKGILYIDSVGFNGRSQCYYFENPADSEKCQKLPFDLKNPYPLLLVNIGSGVSILAVYSKDNYKRVTGTSLGGGTFFGLCCLLTGCTTFEEALEMASRGDSTKVDKLVRDIYGGDYERFGLPGWAVASSFGNMMSKEKREAVSKEDLARATLITITNNIGSIARMCALNENINQVVFVGNFLRINTIAMRLLAYALDYWSKGQLKALFSEHEGYFGAVGALLELLKIP

• Full Name: pantothenate kinase 2
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC009421
• Gene Symbol: PANK2
• Gene ID (NCBI): 80025
• RRID: AB_2159041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BZ23
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Pantothenate kinase 2 (PANK2) is an essential regulatory enzyme in coenzyme A biosynthesis. PANK2 mutations cause pantothenate kinase-associated neurodegeneration (PKAN), which leads to pigmentary retinopathy, progressive dystonia and other abnormalities(PMID:20603201). PanK2 protein is localized to mitochondria of neurons in human brain, distinguishing it from other pantothenate kinases that do not possess mitochondrial-targeting sequences(PMID:15659606 ). It has 4 isoforms produced by alternative splicing and alternative initiation.