PANK2 Polyclonal antibody

PANK2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11001-1-AP

Synonyms

C20orf48, HARP, hPanK2, HSS, NBIA1, PANK2, pantothenate kinase 2, Pantothenic acid kinase 2, PKAN

---

Validation Data Gallery

• 

  WB analysis using 11001-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11001-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11001-1-AP

  IP result of anti-PANK2 (IP:11001-1-AP, 3ug; Detection:11001-1-AP 1:800) with mouse brain tissue lysate 9500ug.

• 

  IHC staining of human lung cancer using 11001-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11001-1-AP (PANK2 antibody) at dilution of 1:10 (under 10x lens).

• 

  IHC staining of human liver using 11001-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11001-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11001-1-AP

  Immunofluorescent analysis of HepG2 cells using 11001-1-AP (PANK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: K-562 cells, human brain tissue, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat brain tissue, rat testis tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:10-1:100
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11001-1-AP targets PANK2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PANK2 fusion protein Ag1413
• Full Name: pantothenate kinase 2
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 59 kDa, 48 kDa
• GenBank Accession Number: BC009421
• Gene Symbol: PANK2
• Gene ID (NCBI): 80025
• RRID: AB_2159041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pantothenate kinase 2 (PANK2) is an essential regulatory enzyme in coenzyme A biosynthesis. PANK2 mutations cause pantothenate kinase-associated neurodegeneration (PKAN), which leads to pigmentary retinopathy, progressive dystonia and other abnormalities(PMID:20603201). PanK2 protein is localized to mitochondria of neurons in human brain, distinguishing it from other pantothenate kinases that do not possess mitochondrial-targeting sequences(PMID:15659606 ). It has 4 isoforms produced by alternative splicing and alternative initiation.