CoraLite®555-conjugated PCK1 Monoclonal antibody

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  IF Staining of HeLa using CL555-66862

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 PCK1 antibody (CL555-66862, Clone: 2F11B5 ) at dilution of 1:200.

PCK1 Monoclonal Antibody for IF/ICC

Cat No. CL555-66862

Clone No.2F11B5

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, pig, human

Applications

IF/ICC

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66862 targets PCK1 in IF/ICC applications and shows reactivity with mouse, rat, pig, human samples.

• Tested Reactivity: mouse, rat, pig, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9720

  Product name: Recombinant human PCK1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 274-622 aa of BC023978

  Sequence: EGEKKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSSEDGEPCAHPNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALKQRISQM

• Full Name: phosphoenolpyruvate carboxykinase 1 (soluble)
• Calculated Molecular Weight: 622 aa, 69 kDa
• Observed Molecular Weight: 65-69 kDa
• GenBank Accession Number: BC023978
• Gene Symbol: PCK1
• Gene ID (NCBI): 5105
• RRID: AB_2934681
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P35558
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).