FcZero-rAb® APC Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using APC-FcA98166-4

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with FcZero-rAb® CoraLite® Plus 488 Anti-Mouse CD3e and 0.1 ug APC Anti-Mouse PD-L1/B7-H1 Rabbit RecAb (APC-FCA98166-4, Clone:242265E6). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using APC-FcA98166-4

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse PD-L1/B7-H1 Rabbit RecAb (APC-FCA98166-4, Clone: 242265E6) (red) or 0.1 ug FcZero-rAb® APC Rabbit IgG Isotype Control Recombinant Antibody (APC-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse PD-L1/B7-H1 Rabbit RecAb (APC-FCA98166-4, Clone: 242265E6) (black, dashed). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

PD-L1/CD274 FcZero-rAb^® Recombinant Antibody for FC

Cat No. APC-FcA98166-4

Clone No.242265E6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Cd274, PD-L1, B7 H1, PD L1, Pdcd1l1

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Tested Applications

• Positive FC detected in: anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA98166-4 targets PD-L1/CD274 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  DNA

• Full Name: CD274 antigen
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: NM_021893.3
• Gene Symbol: PD-L1
• Gene ID (NCBI): 60533
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9EP73
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1) is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cells, and plays an important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL-10 and IFN gamma, in an IL-2-dependent and a PD-1-independent manner.