Tested Applications
| Positive WB detected in | HepG2 cells, HEK293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66119-1-Ig targets PDH E1 Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12556 Product name: Recombinant human PDHA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 41-390 aa of BC002406 Sequence: CDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS Predict reactive species |
| Full Name | pyruvate dehydrogenase (lipoamide) alpha 1 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 39-48 kDa |
| GenBank Accession Number | BC002406 |
| Gene Symbol | PDHA1 |
| Gene ID (NCBI) | 5160 |
| RRID | AB_2881518 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08559 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
| IHC protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
| IP protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
| WB protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-PDHA1 antibody (Cat# 66119-1-Ig) has 13 total citations spanning journals including Science, Nature Metabolism, Nature Communications, Cell Reports, Journal of Cell Biology, Cell Death & Disease, Cancer Metabolism, BMC Cancer, Oxidative Medicine and Cellular Longevity, Translational Oncology, Toxicology and Applied Pharmacology, Immunobiology, and bioRxiv. Research fields cover cancer metabolism, mitochondrial biology, metabolic reprogramming, neurodegeneration, immune evasion, epithelial-mesenchymal transition, and fibrosis.
| Species | Application | Title |
|---|---|---|
Science Boosting neuronal activity-driven mitochondrial DNA transcription improves cognition in aged mice | ||
Nat Metab MAPK signalling-induced phosphorylation and subcellular translocation of PDHE1α promotes tumour immune evasion.
| ||
Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. | ||
Cell Rep A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria. | ||
J Cell Biol PINK1 disables the anti-fission machinery to segregate damaged mitochondria for mitophagy. |
