PDH E1 Alpha Monoclonal antibody

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  WB analysis of HEK293 cells using 66119-1-Ig

  WB result of PDHA1 antibody (66119-1-Ig, 1:30000) with si-control and si-PDHA1 transfected HEK293 cells.

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  WB analysis using 66119-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 66119-1-Ig

  IP result of anti-PDH E1 Alpha (IP:66119-1-Ig, 4ug; Detection:66119-1-Ig 1:10000) with K-562 cells lysate 1820 ug.

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  IHC staining of human liver cancer using 66119-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66119-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66119-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PDH E1 Alpha antibody (66119-1-Ig, Clone: 2B3C10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), PDH E1 Alpha antibody (18068-1-AP, red).

• Featured Product
• KD/KO Validated

PDH E1 Alpha Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 66119-1-Ig

Clone No.2B3C10

Publications(12)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PDH E1α, PDHA1, 2B3C10, EC:1.2.4.1, PDHA

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

66119-1-Ig targets PDH E1 Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12556

  Product name: Recombinant human PDHA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 41-390 aa of BC002406

  Sequence: CDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

• Full Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 39-48 kDa
• GenBank Accession Number: BC002406
• Gene Symbol: PDHA1
• Gene ID (NCBI): 5160
• RRID: AB_2881518
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08559
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
		J Cell Biol; PINK1 disables the anti-fission machinery to segregate damaged mitochondria for mitophagy.; Authors - Kenneth R Pryde
human	WB	Cell Rep; A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria.; Authors - Kenneth Robert Pryde
	WB	Oxid Med Cell Longev; NOX4-Derived ROS Mediates TGF- β 1-Induced Metabolic Reprogramming during Epithelial-Mesenchymal Transition through the PI3K/AKT/HIF-1 α Pathway in Glioblastoma; Authors - Xiangsheng Su
human	WB	Toxicol Appl Pharmacol; Sodium cantharidate induces Apoptosis in breast cancer cells by regulating energy metabolism via the protein phosphatase 5-p53 axis.; Authors - Jin-Long Pang
human	WB	Immunobiology; The mTOR-glycolytic pathway promotes T-cell immunobiology in oral lichen planus.; Authors - Fang Wang