PDH E1 Alpha Monoclonal antibody, PBS Only

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  WB analysis using 66119-1-Ig (same clone as 66119-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK293 cells using 66119-1-Ig (same clone as 66119-1-PBS)

  WB result of PDHA1 antibody (66119-1-Ig, 1:30000) with si-control and si-PDHA1 transfected HEK293 cells. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 66119-1-Ig (same clone as 66119-1-PBS)

  IP result of anti-PDH E1 Alpha (IP:66119-1-Ig, 4ug; Detection:66119-1-Ig 1:10000) with K-562 cells lysate 1820 ug. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66119-1-Ig (same clone as 66119-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PDH E1 Alpha antibody (66119-1-Ig, Clone: 2B3C10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), PDH E1 Alpha antibody (18068-1-AP, red). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PDH E1 Alpha Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 66119-1-PBS

Clone No.2B3C10

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

PDH E1α, PDHA1, 2B3C10, EC:1.2.4.1, PDHA

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Product Information

66119-1-PBS targets PDH E1 Alpha in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12556

  Product name: Recombinant human PDHA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 41-390 aa of BC002406

  Sequence: CDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

• Full Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 39-48 kDa
• GenBank Accession Number: BC002406
• Gene Symbol: PDHA1
• Gene ID (NCBI): 5160
• RRID: AB_2881518
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08559
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).