Tested Applications
| Positive WB detected in | HeLa cells, HEK293 cells, mouse brain tissue, rat brain tissue, rat kidney tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18068-1-AP targets PDH E1 Alpha in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12701 Product name: Recombinant human PDHA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-390 aa of BC002406 Sequence: CDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS Predict reactive species |
| Full Name | pyruvate dehydrogenase (lipoamide) alpha 1 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 39-48 kDa |
| GenBank Accession Number | BC002406 |
| Gene Symbol | PDHA1 |
| Gene ID (NCBI) | 5160 |
| RRID | AB_2162931 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08559 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing with the molecular mass of 39-48 kDa. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDH E1 Alpha antibody 18068-1-AP | Download protocol |
| IHC protocol for PDH E1 Alpha antibody 18068-1-AP | Download protocol |
| IP protocol for PDH E1 Alpha antibody 18068-1-AP | Download protocol |
| WB protocol for PDH E1 Alpha antibody 18068-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PDHA1 (Cat# 18068-1-AP) has 118 citations published in journals including Nature, Cell Metabolism, Nature Metabolism, Nature Communications, Cell Stem Cell, Nature Cell Biology, Neuron, EMBO Journal, Oncogene, and Cell Reports. Research fields span metabolic reprogramming, mitochondrial biology, cancer metabolism, cardiac and neuronal metabolism, immunology, ferroptosis, cuproptosis, diabetes, and aging.
| Species | Application | Title |
|---|---|---|
Imeta Clinical features and molecular landscape of cuproptosis signature-related molecular subtype in gastric cancer | ||
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Cell Metab Semaglutide ameliorates osteoarthritis progression through a weight loss-independent metabolic restoration mechanism. | ||
Cell Metab Environment Impacts the Metabolic Dependencies of Ras-Driven Non-Small Cell Lung Cancer.
| ||
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation |
Reviews
What customers say
All three reviews are for Western Blot applications at a 1:1000 dilution. Users report clear, strong band detection across multiple sample types, including human liver cells, brain tissue, and whole tissue/cell homogenates from human, mouse, and rat. One reviewer noted good detection with as little as 30 µg of protein, while another provided a detailed protocol using a 4–12% gradient gel and Odyssey imaging. Overall, the antibody is well-regarded for its sensitivity and consistent performance across species and tissue types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
kes (Verified Customer) (01-17-2022) | I used it for WB(1:1000) and got nice bands.
 |
David (Verified Customer) (09-03-2019) | Good detection with 30µg of protein and dilution at 1/1000
 |
Svitlana (Verified Customer) (11-02-2017) | Loading:per well 15 ug/ulGradient gel 4-12%Transfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hImaging:Odyssey CLx Imaging
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