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Tested Applications
| Positive WB detected in | HeLa cells, THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
30560-1-AP targets PGLS in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30836 Product name: Recombinant human PGLS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-131 aa of BC014006 Sequence: MAAPAPGLISVFSSSQELGAALAQLVAQRAACCLAGARARFALGLSGGSLVSMLARELPAAVAPAGPASLARWTLGFCDERLVPFDHAESTYGLYRTHLLSRLPIPESQVITINPELPVEEAAEDYAKKLR Predict reactive species |
| Full Name | 6-phosphogluconolactonase |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC014006 |
| Gene Symbol | PGLS |
| Gene ID (NCBI) | 25796 |
| RRID | AB_3086363 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95336 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
6-phosphogluconolactonase (PGLS) ,the key enzyme of pentose phosphate pathway, was aberrantly highly expressed in various cancers, including cervical cancer, breast cancer, glioblastomas, and hepatic cancer, etc.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PGLS antibody 30560-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
