PHLDA1 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• PHLDA1 Polyclonal antibody, PBS Only (18263-1-PBS)

• 

  WB analysis using 18263-1-AP (same clone as 18263-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18263-1-AP (PHLDA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 18263-1-AP (same clone as 18263-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18263-1-AP (PHLDA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 18263-1-AP (same clone as 18263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18263-1-AP (PHLDA1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A375 using 18263-1-AP (same clone as 18263-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using PHLDA1 antibody (18263-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PHLDA1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 18263-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Apoptosis-associated nuclear protein, DT1P1B11, PHRIP, Pleckstrin homology-like domain family A member 1, PQ-rich protein

---

Product Information

18263-1-PBS targets PHLDA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13125

  Product name: Recombinant human PHLDA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-259 aa of BC018929

  Sequence: MLESSGCKALKEGVLEKRSDGLLQLWKKKCCILTEEGLLLIPPKQLQHQQQQQQQQQQQQQQPGQGPAEPSQPSGPAVASLEPPVKLKELHFSNMKTVDCVERKGKYMYFTVVMAEGKEIDFRCPQDQGWNAEITLQMVQYKNRQAILAVKSTRQKQQHLVQQQPPSQPQPQPQLQPQPQPQPQPQPQPQSQPQPQPQPKPQPQQLHPYPHPHPHPHSHPHSHPHPHPHPHPHQIPHPHPQPHSQPHGHRLLRSTSNSA

• Full Name: pleckstrin homology-like domain, family A, member 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC018929
• Gene Symbol: PHLDA1
• Gene ID (NCBI): 22822
• RRID: AB_2163284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WV24
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PHLDA1, also known as PHRIP and TDAG51, is a multifunctional protein involved in various biological processes. It can induce apoptosis in various cell types, including T cells, hippocampal cells, endothelial cells, melanoma cells, and mouse embryonic fibroblasts(PMID: 30207029). PHLDA1 plays a role in inhibiting growth factor signaling and has been implicated in tumor suppression through its ability to repress Akt activity by binding to phosphatidylinositol (PIP) lipids(PMID: 36142223).