PJA1 Polyclonal antibody, PBS Only

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  WB analysis using 17687-1-AP (same clone as 17687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 17687-1-AP (same clone as 17687-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

  IP result of anti-PJA1 (IP:17687-1-AP, 3ug; Detection:17687-1-AP 1:300) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PJA1 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 17687-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase Praja-1, EC:2.3.2.27, praja ring finger 1, Praja1, RING finger protein 70

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Product Information

17687-1-PBS targets PJA1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11820

  Product name: Recombinant human PJA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 294-643 aa of BC075803

  Sequence: ANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

• Full Name: praja ring finger 1
• Calculated Molecular Weight: 643 aa, 71 kDa
• Observed Molecular Weight: 71-80 kDa
• GenBank Accession Number: BC075803
• Gene Symbol: PJA1
• Gene ID (NCBI): 64219
• RRID: AB_2252483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NG27
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.